So, let's say you did an awesome experiment here using HeLa cells and you're looking at this protein, LDHB, and you're just validating and make sure it works. Let's go into western blotting specifically. So, these are more broader reasons for why controls are needed. It also allows you to compare data with your results, other lab members, as well as published data. And as well as minimize the variables that may be due to human error or environmental changes or what-have-you. Otherwise, there may be artifacts, background signal that make it challenging to understand whether this is a real sample or not. Why are controls in antibody application so important? So, first of all, it ensures that your results are biologically real. So, now, let's get to the meat and potatoes of this. And you can see this graph that's got a steep slope, so we're really excited to see that we're making a big impact in helping you guys make the next great discovery. So, below here, you can see some cover stories of studies that have used our antibodies, and we've been cited over 70,000 times in publications worldwide. Our CoraLite brand of fluorescent-dye conjugated antibodies, neutralizing antibodies, and prestained protein markers/ladders.Īnd so, for us, it's really about helping you guys, and so we really love to celebrate the success our scientists have had, and we're very proud of the great discoveries they've made. We also sell a line of HumanKine, human cell-expressed cytokines, and growth factors for use in cell and gene therapies. We have more than 200 ELISA kits available. We have 2,500 targets that have validated by knockout or knocked down validation. We have antibodies against 13,000 human targets. So, a little bit more about our products. And as well as being able to provide all of our original validation data, including our raw films, and we have great stock inventory as a result of being able to control everything. And so, the consistency may not be the same to you. And so, in addition, as I said before, this gives us a little bit more lot-to-lot consistency because reseller D may get it from manufacturer D now, but they may get it from manufacturer C later. Everything that we make is straight from ourselves, and it is completely unique. And so, you don't have to worry about this kind of a web of purchases in between these different vendors. So, at Proteintech, we sell only our stuff, and we sell it directly to you. But they're relabeled and sent in such a way that you don't really know beforehand that you bought four vials of the same exact thing. So, what this means is that to you, the customer, you might be getting an antibody from A, B, C, and D, but they could all be coming from manufacturer A, for instance. And sometimes the resellers sell that stuff to you, or they sell between themselves, or they sell it to other manufacturers. ![]() Our slogan is, "From our bench to your bench," and so, what do we mean by that? So, in terms of how the antibody industry is now, we have a bunch of different manufacturers, as denoted here, and they sell to resellers. Our products have been cited over 70,000 times in publications worldwide.Īnd we also love to support the scientific community with mentorship awards, travel grants, workshops, and more to help you guys become the 70,001st publication. We have five sites globally with full product inventory. We recently received ISO certification, which just means that we have a process in place for keeping things at a very high quality and reproducibility, and it also allows us to sell GMP-grade products. So, at Proteintech, one of the major things that we do is all of our products are manufactured and validated in-house, so that gives us a lot of quality control, as well as to be able to really know everything about the antibody that you're purchasing. So, for those of you who are not familiar with Proteintech, I just wanted to give a short intro here. So, in terms of our agenda today, what we're going to work on is why do you run controls in the first place, some considerations when choosing controls, going through a few popular controls, and then ending with protein ladders, one final control that you need in your experiments. I've been working on a lot of the technical content for the last five years, and I'm very excited today, to tell you more about how to use controls and which controls are best for your particular experiments. I'm the scientific officer for Proteintech Group. Welcome everybody to this webinar on Understanding Your Controls for Western Blotting.
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